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Gurugrah Questions: Your Source for Thoughtful Queries, Your Gateway to Enlightening Answers.
Sequence No.
BIO-868
Recombinant DNA technology is of great importance in the field of medicine, with the help of a flow chart, show now this technology has been used in preparing genetically engineered human insulins.
Or
How Eli Lilly, an american company produced insulin by recombinant DNA technology?
Answer:
To control the infection of meloidogyne incognita a novel strategy has been used which is RNAi.
- Firstly we isolate those genes from nematode which are important for the survival and introduce into Agrobacterium vector to form rDNA.
- This rDNA how infected to the plant cell, where it produce both sense and anti - sense RNA in plant cell.
- We ensurely take only those gene from M. incognita that have ability to form dsRNA.
- dsRNA formed in plant cell, cut by dicer enzyme into small dsRNA, having RNAi specific sequence.
- When nematode eat such cells, these RNAi silenced the mRNA of nematode and stop protein translation.
- Without protein or enzyme nematode get dies.
Reporting Question Sequence No.
BIO-868
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